Gary Markey

Education

2015 – to date               Maynooth University, Maynooth, Co.Kildare.

                                    B.Sc. (Honours) Biomedical Science

                                    Expected Result: Honours 2.1

Thesis title: Hypoxic mechanisms regulating epithelial regeneration in allergic oesophagitis

Laboratory Skills & Instrumentation 

• General: Clinical sample handling, Aseptic technique, cell culture, Centrifugation safety training, Data analysis, Literature researching, Presentation skills, Report writing, Documentation and Quality Management Systems.

Laboratory Experience

• Organic and Inorganic Chemistry,
• Biomedical research lab (Institute of Immunology, Human health institute, Maynooth University),
• Biopsy handling, Immunohistochemistry, FISH,
• Animal specimen dissection and tissue/organ harvesting and preparation
• Cell / tissue culture,
• Tissue staining,
• Microtome applications,   
• RT PCR,
• Data extrapolation & analysis,

Instrumentation and Analytical Experience

• Volumetric Titrations
• Atomic Absorption Spectroscopy (AA)
• Infrared Spectroscopy
• Ultraviolet and Visible Absorption Spectroscopy (UV-Vis)
• Mass Spectrometry & Proteomic analysis
• HPLC/MS/MS.
• Western Blotting
• Microbiological and Immunological analysis
• Immunohistochemistry.
• Toxicological analysis
• Polymerase Chain Reaction (PCR)
• Thin layer Chromatography (TLC), Gas Chromatography (GC),

       High Performance Liquid Chromatography (HPLC).

Computer

• Computer Applications - extensive experience with spreadsheet and graphical analysis packages, Microsoft Office suite (WORD, EXCEL, POWERPOINT),

• Web - Good understanding of Web, Website design using Microsoft Publisher, Journal Databases.

Instructional

• • • Private tutor to students in Leaving Cert Biology
    • Lab Demonstrations to students during Maynooth University Laboratory open nights
    • Open days (November 2017- Present)

Vacation Employment

• June – September 2018    Researcher (SPUR Scholarship) – Allergy Inflammation & Remodelling Laboratory, Maynooth University. Optimisation of microRNA detection by in situ hybridisation in Eosinophilic Oesophagitis (EoE)

• Ensure EoE clinical biopsy samples were suitable for analysis.
• Poster presentation of our research findings at a research symposium at Maynooth University.
• In situ hybridisation with immunofluorescence on patient tissue samples.
• Statistical analysis and extrapolation of data.
• Histopathological analysis and comparison of diseased tissue vs healthy.
• Microscopic image acquisition and quantitative analysis.
• Mammalian tissue culture   
• September to May 2017: Maynooth Student’s Union (MSU)

                                       Operations team (Office)

• Responsible for collecting and sending post
• Provide efficient service to students with any enquiries
• Ensure the meeting rooms are kept in good condition
• Issue students with student Leap cards
• Support staff members with paperwork and other clerical duties
• Comfortable working in busy environment and to tight deadlines

• June 2017 to August 2017: Cineworld Cinema, Parnell Street, Dublin 1

                                            Team Member

• Customer service & stock rotation
• Manual handling
• Cash handling
• Monitored the fire exits every morning and evening

Interests and Achievements

University:  Member – Biology Society, Maynooth University

• Volunteered as a lab demonstrator to school students during the Biology departmental open day.

• Volunteered as representative for the Biology Department giving school students insight on the variety of biological / biomedical research undertaken at Maynooth University.

Scholarship: Maynooth Summer Research Scholarship (SPUR):

• Awarded a competitive €1500 SPUR scholarship to undertake Biomedical research into   Eosinophilic Esophagitis under mentorship of Dr Joanne Masterson.
• Experience in translational clinical research setting by working in one of the
• Institute of Immunology’s labs at Maynooth University

Vacation Employment

• June – September 2018    Researcher (SPUR Scholarship) – Allergy Inflammation & Remodelling Laboratory, Maynooth University. Optimisation of microRNA detection by in situ hybridisation in Eosinophilic Oesophagitis (EoE)

• Ensure EoE clinical biopsy samples were suitable for analysis.
• Poster presentation of our research findings at a research symposium at Maynooth University.
• In situ hybridisation with immunofluorescence on patient tissue samples.
• Statistical analysis and extrapolation of data.
• Histopathological analysis and comparison of diseased tissue vs healthy.
• Microscopic image acquisition and quantitative analysis.
• Mammalian tissue culture   

September to May 2017: Maynooth Student’s Union (MSU)

                                       Operations team (Office)

• Responsible for collecting and sending post
• Provide efficient service to students with any enquiries
• Ensure the meeting rooms are kept in good condition
• Issue students with student Leap cards
• Support staff members with paperwork and other clerical duties
• Comfortable working in busy environment and to tight deadlines

June 2017 to August 2017: Cineworld Cinema, Parnell Street, Dublin 1

                                            Team Member

• Customer service & stock rotation
• Manual handling
• Cash handling
• Monitored the fire exits every morning and evening

Scholarship: Maynooth Summer Research Scholarship (SPUR):

• Awarded a competitive €1500 SPUR scholarship to undertake Biomedical research into   Eosinophilic Esophagitis under mentorship of Dr Joanne Masterson.
• Experience in translational clinical research setting by working in one of the Institute of Immunology’s labs at Maynooth University

• June – September 2018    Researcher (SPUR Scholarship) – Allergy Inflammation & Remodelling Laboratory, Maynooth University. Optimisation of microRNA detection by in situ hybridisation in Eosinophilic Oesophagitis (EoE)
• Ensure EoE clinical biopsy samples were suitable for analysis.
• Poster presentation of our research findings at a research symposium at Maynooth University.
• In situ hybridisation with immunofluorescence on patient tissue samples.
• Statistical analysis and extrapolation of data.
• Histopathological analysis and comparison of diseased tissue vs healthy.
• Microscopic image acquisition and quantitative analysis.
• Mammalian tissue culture   

Education

2015 – to date               Maynooth University, Maynooth, Co.Kildare.

                                    B.Sc. (Honours) Biomedical Science

                                    Expected Result: Honours 2.1

Thesis title: Hypoxic mechanisms regulating epithelial regeneration in allergic oesophagitis

I have presented my research at resarch symposiums as well as oral presentations as part of my unegraduate degree and as part of my schoarship. A sample of my research poster may be viwed on my LinkedIn account https://www.linkedin.com/in/gary-markey-856880127/