Y chromosome Microdeletion Kit was designed to amplify 3 regions of the azospermia factors (AZF) gene (AZFa, AZFb, and AZFc) using 6 sequence-tagged sites (STS). The kit is for use on human genomic DNA samples and will provide a qualitative assessment of microdeltion status. According to best practice guidelines for molecular diagnosis of Y-chromosome microdeletions formulated by (the European Academy of Andrology (EAA) and the European Molecular Genetics Quality Network(EMQN). The kit is performed by two sets of multiplex PCR amplification of genomic DNA, using the Zinc finger protein, X-linked (ZFX)/Zinc finger protein, Y-linked (ZFY) and sex-determining region of the Y chromosome (SRY) as internal PCR control, STSs in each AZF region. These STSs include: For AZFa: sY84, sY86 For AZFb: sY127, sY134 For AZFc: sY254, sY255 (both in the DAZ gene)
New Y Chromosomal microdeletions lyophilized real time PCR kit is available now, only 2 tubes to detect six Sequence Taq Site(STS) microdeletions in AZFa,AZFb,AZFc area.please send your inquiry to [email protected], thanks.