Klaus Wiemer PhD (Lab Director POMA Fertility) discusses the benefits of continuous non-invasive pH monitoring and shares clinical practices that were optimized by POMA Fertility as a result of their utilization of the SAFE Sens system within their incubators including:

• Longer equilibration times for dish preparation
• Increased oil overlay to prevent evaporation

Steven Geelhood (SAFE Sens Project Manager) then shared data collected from labs utilizing the SAFE Sens continuous pH monitory system.   Graphs were presented and analyzed for the following scenarios wherein cell culture conditions were manipulated thus causing changes to the pH over time.  

• Initial equilibration of media with time scale 
• Equilibrated media with or without oil
• CO2 set point changes
•  Effect of loss of CO2 

Finally, Dr. Wiemer and Mr. Geelhood answered questions from live participant.

http://www.safesens.com/webinar/real-world-best-practices-in-ivf-cell-culturing-continuous-ph-monitoring/