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Vijay Konala

 
Full Name: Vijay Konala
Position Sought: Embryologist/ Research scientist
Primary Language: English
Address: Bangalore
Karnataka
India
530096
Telephone: +917893921414
E-mail: Contact Vijay
 Profile and objectives
15+ Work experience in the area of human embryology and stem cell biology
  Main resume

Vijay B Reddy Konala

           

                                                                                                        

Present address: Flat no: 302, H.Heights, Virupakshapura,        Permanent address # 68, MVP Colony, Vishakhapatnam,

Sahakaranagar, Bangalore- 560 097                                            Andhra Pradesh, India- 530 022

 

Ph: +91-7893921414 Email:

 

LinkedIn: https://www.linkedin.com/in/vijay-bhaskar-reddy-konala-201b3338/

 

Career Highlights

 

 

A Biotechnology professional with more than 10 years of experience into Cell biology, Nucleofection technology, Epidermal & Cornea 3D models, IVF Technology, Primary cells and Cell lines.

Team Player, Good interpersonal skills, Growth mindset and independently handle multiple projects.

Diverse experience in the area of Cell Biology in Domestic & Multinational R & D organizations, including International work experience in UAE.

 

Major accomplishments & publications:

 

PUBLICATIONS

 

Papers

 

  • The current landscape of the mesenchymal stromal cell secretome: A new paradigm for cell-free regeneration, Vijay Bhaskar Reddy Konala*, Murali Krishna Mamidi, Ramesh Bhonde,Anjan Kumar Das, Radhika Pochampally and Rajarshi Pal, Cytotherapy. 2016 Jan; 18(1): 13–24.
  • Sequential cultivation  of human  epidermal keratinocytes  and  dermal mesenchymal  like stromal cells in vitro, Shyam Mahabal*, Vijay Bhaskar Reddy Konala*, Murali Krishna Mamidi1, Mohammad Mahboob Kanafi, Suniti Mishra, Krupa Shankar, Rajarshi Pal and Ramesh Bhonde, Cytotechnology , 2016 Aug;68(4):1009-18
  • Assessment of drug induced developmental toxicity using human embryonic stem cells, Ashish Mehta*, Vijay Bhaskar R. Konala, Aparna Khanna, Anish S. Majumdar, Cell Biology International, 32 (2008) 1412-1424.

 

Book Chapter

 

  • Chapter entitled ‘Embryonic stem cells: Development and Characterization’ Vijay Bhaskar R. Konala, Villoo Morawala-Patell, and Aparna Khanna*, Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration, Wiley publication.

Patent

 

  • In vitro assay methods for classifying embryo toxicity of compounds by using hES cells (US12043899). Ashish Mehta, Vijay Bhaskar Reddy and Aparna Khanna. March 2007

 

 

 

 

Academic & Professional Qualification

  • Masters of Science (MSc) in Microbiology, Andhra Pradesh University, Vishakhapatnam, India
  • Bachelors of Science (BSc) in Microbiology, Andhra Pradesh University, Vishakhapatnam, India

Work Experience

 

 

 

Eyestem Research Private Limited                                         Senior Research Associate

http://www.eyestem.com                                                           Jan 2018 to till to date

 

 

 

  • Human iPSC derivation and characterization from human PBMCs
  • Human iPSC differentiation in to RPE and PR cell types

 

 

 

Ova Science, UAE                                                                   Scientist – Clinical & Global Operations

http://www.ovascience.com                                                       Dec 2015- Nov 2017

 

 

 

  • Augment Therapy:
    • Designed to enrich Egg health.
    • Isolation of mitochondria from the ovarian tissue and delivering back to embryology lab.
  • Ova Prime therapy: This therapy aimed to rejenuvate ovarian reservoir. 
  • Isolation of Egg precursor cells from ovarian tissue & injecting back to ovaries with the help of surgeon.
  • Involved in the antibody titration studies, process optimization studies for flow cytometry and environmental monitoring.

 

Lonza India Pvt Ltd                                                                                        Scientist – Bio Services

http://www.lonza.com/                                                                                      Sep 2010 – Nov 2015

 

 

  • Stable cell line development by using Nucleofection technology
  • Establishment of 3D Cornea and In vitro epidermal models as part of toxicity studies
  • Primary Cell Isolation: Keratinocytes, Melanocytes, Fibroblasts, Synovial fibroblasts and Follicle dermal papilla cells.
  • Stem cell characterization- Morphology, molecular, cellular and functional analysis.
  • Nucleofection protocol optimization for the primary cells and cell lines.
  • HUVEC isolation and optimization of tube formation protocol

 




 

ANSA Research Pvt Ltd, Bangalore                           Senior Executive – Operations & Research

http://ansaresearch.com/                                                 November 2009 to June 2010

  • Isolation, maintenance and differentiation of Mesenchymal Stem Cells (MSCs) from Bone Marrow (BM), Wharton’s Jelly (WJ), Adipose and Dental Pulp (DP)
  • Large-scale production of hMSCs at 5 & 10 cell stack level at cGMP conditions.
  • Involved in the tumor repository set up and responsible for making RNA bank.

 




 

Avesthagen Ltd, Bangalore                                                                 Scientist – ESC Group

www.avesthagen.com                                                                           August 2008 to October 2009

           

  • Project awarded from DBT entitled ‘Differentiation of human embryonic stem cells in to hepatocyte like cells to screen the bio actives for hepatotoxicity under SIBRI scheme.
  • Culture and maintenance of hESC lines (BJNhem19 and BJNhem20) at JNCASR.

    

Reliance Life Sciences P Ltd, Mumbai                                                 Research Fellow – ESC Group

www.rellife.com                                                                                   Jan 2006 to July 2008

 

 

  • Derivation of Mouse  Embryonic Fibroblast cells (MEF)
  • Culturing and maintenance of human embryonic stem  cell lines  (BG01, BG01V, and   Relli®CellhES1),   human  carcinoma  cell   lines   (NTERA,   HepG2),   human embryonic and foreskin fibroblast cells (HEF & HFF)
  • Development of molecular end   point   based   embryo toxicity  assays   to  analyze teratogens using  human embryonic stem cells (hESC)
  • Differentiation of hESC-  derived  hepatocytes employing  different strategies in order to use them  as a model to screen drugs based  on hepatotoxicity.
  • Characterization of hESC- derived cells by Immunofluorescence (IFL), Western Blot (WB), Reverse Transcriptase Polymerase Chain Reaction  (RT-PCR),  Fluorescence Activated Cell Sorting  (FACS), Functional assays  and  Real time Taqman assays  (Q- PCR/TLDA) by using  various markers.

 

 

Sri Satya IVF Clinic    , Vishakhapatnam                                                       Embryologist

                                                                                                                        June 2001 to December 2005   

  • Oocyte pickup and grading
  • In Vitro Fertilization & Embryo Transfer (IVF & ET)
  • Intra  Cytoplasmic Sperm  Injection  (ICSI)
  • Blastocyst culture by open and closed cultures
  • Sperm washing and processing to enhance the sperm activity for IUI
  • Semen evaluation, cryopreservation and thawing
  • Management of donor sperm cryobank,  quality control and patient counselling

 

ADDITIONAL IFORMATION

 

Published abstracts

 

  •   Scientific paper entitled “ Tube Formation Assay with Primary Human Umbilical Vein Endothelial Cells” Renu Kapoor, Vijay Reddy and Preeti Kapoor, available at Lonza website

 

  •   Abstract presented entitled ‘Embryonic stem cells as a tool for predictive toxicology’ Ashish Mehta, Vijay Bhaskar Reddy and Aparna Khanna*, at ICMR (Indian Council of Medical Research) 29th January -1st February, 2007. Bangalore, India.

 

  • Presented poster entitled ‘Evaluation of a Candidate Set of Genes in Drug Induced Toxicity Using Human Embryonic Stem Cells by Taqman Low Density Arrays’ Konala Vijay Bhaskar R*, Mehta Ashish and Khanna Aparna, at International Cell Biology Conference’, organized by Banaras Hindu University, Varanasi, India.

 

  • Abstract presented entitled ‘Use of human embryonic stem cells to assess developmental toxicity of biologically active substances in vitro’ Ashish Mehta*, Vijay Bhaskar R.Konala, Anish S Majumdar, Aparna Khanna, 6th annual meeting of the International Society for Stem Cell Research (ISSCR), Philadelphia, PA, USA, June 11-14, 2008.

 

Workshops attended

 

  • Attended the ‘International Pre-Congress Workshop on Embryology’, November 2003, Mumbai, INDIA

 

  • Attended the ‘Symposium and Workshop on Advanced Gamete Micromanipulation Techniques’, conducted by University of Calcutta, INDIA.

 

Stem Cell Workshop

 

  • Selected  and  participated in “hands on stem cell workshop”, organized  by the British High Commission and Department of Science and Technology, Govt. of India in NCBS at Bangalore from April 4th -11th 2005

 

  • Part of the organization committee and faculty for the “international workshop on the human embryonic stem cells & Symposium” organized from February 21st to29th 2006 and February 5th to 9th 2007, Reliance Life Sciences Pvt. Ltd., Navi-Mumbai-4001701 Maharashtra, INDIA

 

Awards

 

  • Received best employee award (Lonza) for the 2011-second quarter towards the optimization of proliferating cell shipment to our customers across country.

 

Personal Information

 

Date of Birth                                                    11 May 1979

Nationality & Passport                                       India

Languages known                                             English, Hindi & Telugu

                                                                                               

References

Dr. Aparna Khanna                                                         David O’Donnell

Dean, School of science,                                                 Director, Cell Processing

SVKM’s NMIMS University,                                        OvaScience, Inc.

Mumbai- 400 057                                                           9 Fourth Avenue, Waltham, MA 02451, USA

Email: u                              Mobile : +1-978-210-4440

Cell: +91 9324292883                                                

Vijay B Reddy Konala

           

                                                                                                        

Present address: Flat no: 302, H.Heights, Virupakshapura,        Permanent address # 68, MVP Colony, Vishakhapatnam,

Sahakaranagar, Bangalore- 560 097                                            Andhra Pradesh, India- 530 022

 

Ph: +91-7893921414 Email:

 

LinkedIn: https://www.linkedin.com/in/vijay-bhaskar-reddy-konala-201b3338/

 

Career Highlights

 

 

 

A Biotechnology professional with more than 10 years of experience into Cell biology, Nucleofection technology, Epidermal & Cornea 3D models, IVF Technology, Primary cells and Cell lines.

Team Player, Good interpersonal skills, Growth mindset and independently handle multiple projects.

Diverse experience in the area of Cell Biology in Domestic & Multinational R & D organizations, including International work experience in UAE.

 

Major accomplishments & publications:

 

PUBLICATIONS

 

Papers

 

  • The current landscape of the mesenchymal stromal cell secretome: A new paradigm for cell-free regeneration, Vijay Bhaskar Reddy Konala*, Murali Krishna Mamidi, Ramesh Bhonde,Anjan Kumar Das, Radhika Pochampally and Rajarshi Pal, Cytotherapy. 2016 Jan; 18(1): 13–24.
  • Sequential cultivation  of human  epidermal keratinocytes  and  dermal mesenchymal  like stromal cells in vitro, Shyam Mahabal*, Vijay Bhaskar Reddy Konala*, Murali Krishna Mamidi1, Mohammad Mahboob Kanafi, Suniti Mishra, Krupa Shankar, Rajarshi Pal and Ramesh Bhonde, Cytotechnology , 2016 Aug;68(4):1009-18
  • Assessment of drug induced developmental toxicity using human embryonic stem cells, Ashish Mehta*, Vijay Bhaskar R. Konala, Aparna Khanna, Anish S. Majumdar, Cell Biology International, 32 (2008) 1412-1424.

 

Book Chapter

 

  • Chapter entitled ‘Embryonic stem cells: Development and Characterization’ Vijay Bhaskar R. Konala, Villoo Morawala-Patell, and Aparna Khanna*, Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration, Wiley publication.

Patent

 

  • In vitro assay methods for classifying embryo toxicity of compounds by using hES cells (US12043899). Ashish Mehta, Vijay Bhaskar Reddy and Aparna Khanna. March 2007

 

 

 

 

Academic & Professional Qualification

  • Masters of Science (MSc) in Microbiology, Andhra Pradesh University, Vishakhapatnam, India
  • Bachelors of Science (BSc) in Microbiology, Andhra Pradesh University, Vishakhapatnam, India

Work Experience

 

 

 

Eyestem Research Private Limited                                         Senior Research Associate

http://www.eyestem.com                                                           Jan 2018 to till to date

 

 

 

  • Human iPSC derivation and characterization from human PBMCs
  • Human iPSC differentiation in to RPE and PR cell types

 

 

 

Ova Science, UAE                                                                   Scientist – Clinical & Global Operations

http://www.ovascience.com                                                       Dec 2015- Nov 2017

 

 

 

  • Augment Therapy:
    • Designed to enrich Egg health.
    • Isolation of mitochondria from the ovarian tissue and delivering back to embryology lab.
  • Ova Prime therapy: This therapy aimed to rejenuvate ovarian reservoir. 

  • Isolation of Egg precursor cells from ovarian tissue & injecting back to ovaries with the help of surgeon.
  • Involved in the antibody titration studies, process optimization studies for flow cytometry and environmental monitoring.

 

Lonza India Pvt Ltd                                                                                        Scientist – Bio Services

http://www.lonza.com/                                                                                      Sep 2010 – Nov 2015

 

 

  • Stable cell line development by using Nucleofection technology
  • Establishment of 3D Cornea and In vitro epidermal models as part of toxicity studies
  • Primary Cell Isolation: Keratinocytes, Melanocytes, Fibroblasts, Synovial fibroblasts and Follicle dermal papilla cells.
  • Stem cell characterization- Morphology, molecular, cellular and functional analysis.
  • Nucleofection protocol optimization for the primary cells and cell lines.
  • HUVEC isolation and optimization of tube formation protocol

 

 

 

 

 

ANSA Research Pvt Ltd, Bangalore                           Senior Executive – Operations & Research

http://ansaresearch.com/                                                 November 2009 to June 2010

  • Isolation, maintenance and differentiation of Mesenchymal Stem Cells (MSCs) from Bone Marrow (BM), Wharton’s Jelly (WJ), Adipose and Dental Pulp (DP)
  • Large-scale production of hMSCs at 5 & 10 cell stack level at cGMP conditions.
  • Involved in the tumor repository set up and responsible for making RNA bank.

 

 

 

 

 

Avesthagen Ltd, Bangalore                                                                 Scientist – ESC Group

www.avesthagen.com                                                                           August 2008 to October 2009

           

  • Project awarded from DBT entitled ‘Differentiation of human embryonic stem cells in to hepatocyte like cells to screen the bio actives for hepatotoxicity under SIBRI scheme.
  • Culture and maintenance of hESC lines (BJNhem19 and BJNhem20) at JNCASR.

    

Reliance Life Sciences P Ltd, Mumbai                                                 Research Fellow – ESC Group

www.rellife.com                                                                                   Jan 2006 to July 2008

 

 

  • Derivation of Mouse  Embryonic Fibroblast cells (MEF)
  • Culturing and maintenance of human embryonic stem  cell lines  (BG01, BG01V, and   Relli®CellhES1),   human  carcinoma  cell   lines   (NTERA,   HepG2),   human embryonic and foreskin fibroblast cells (HEF & HFF)
  • Development of molecular end   point   based   embryo toxicity  assays   to  analyze teratogens using  human embryonic stem cells (hESC)
  • Differentiation of hESC-  derived  hepatocytes employing  different strategies in order to use them  as a model to screen drugs based  on hepatotoxicity.
  • Characterization of hESC- derived cells by Immunofluorescence (IFL), Western Blot (WB), Reverse Transcriptase Polymerase Chain Reaction  (RT-PCR),  Fluorescence Activated Cell Sorting  (FACS), Functional assays  and  Real time Taqman assays  (Q- PCR/TLDA) by using  various markers.

 

 

Sri Satya IVF Clinic    , Vishakhapatnam                                                       Embryologist

                                                                                                                        June 2001 to December 2005   

  • Oocyte pickup and grading
  • In Vitro Fertilization & Embryo Transfer (IVF & ET)
  • Intra  Cytoplasmic Sperm  Injection  (ICSI)
  • Blastocyst culture by open and closed cultures
  • Sperm washing and processing to enhance the sperm activity for IUI
  • Semen evaluation, cryopreservation and thawing
  • Management of donor sperm cryobank,  quality control and patient counselling

 

ADDITIONAL IFORMATION

 

Published abstracts

 

  •   Scientific paper entitled “ Tube Formation Assay with Primary Human Umbilical Vein Endothelial Cells” Renu Kapoor, Vijay Reddy and Preeti Kapoor, available at Lonza website

 

  •   Abstract presented entitled ‘Embryonic stem cells as a tool for predictive toxicology’ Ashish Mehta, Vijay Bhaskar Reddy and Aparna Khanna*, at ICMR (Indian Council of Medical Research) 29th January -1st February, 2007. Bangalore, India.

 

  • Presented poster entitled ‘Evaluation of a Candidate Set of Genes in Drug Induced Toxicity Using Human Embryonic Stem Cells by Taqman Low Density Arrays’ Konala Vijay Bhaskar R*, Mehta Ashish and Khanna Aparna, at International Cell Biology Conference’, organized by Banaras Hindu University, Varanasi, India.

 

  • Abstract presented entitled ‘Use of human embryonic stem cells to assess developmental toxicity of biologically active substances in vitro’ Ashish Mehta*, Vijay Bhaskar R.Konala, Anish S Majumdar, Aparna Khanna, 6th annual meeting of the International Society for Stem Cell Research (ISSCR), Philadelphia, PA, USA, June 11-14, 2008.

 

Workshops attended

 

  • Attended the ‘International Pre-Congress Workshop on Embryology’, November 2003, Mumbai, INDIA

 

  • Attended the ‘Symposium and Workshop on Advanced Gamete Micromanipulation Techniques’, conducted by University of Calcutta, INDIA.

 

Stem Cell Workshop

 

  • Selected  and  participated in “hands on stem cell workshop”, organized  by the British High Commission and Department of Science and Technology, Govt. of India in NCBS at Bangalore from April 4th -11th 2005

 

  • Part of the organization committee and faculty for the “international workshop on the human embryonic stem cells & Symposium” organized from February 21st to29th 2006 and February 5th to 9th 2007, Reliance Life Sciences Pvt. Ltd., Navi-Mumbai-4001701 Maharashtra, INDIA

 

Awards

 

  • Received best employee award (Lonza) for the 2011-second quarter towards the optimization of proliferating cell shipment to our customers across country.

 

Personal Information

 

Date of Birth                                                    11 May 1979

Nationality & Passport                                       India

Languages known                                             English, Hindi & Telugu

                                                                                               

References

Dr. Aparna Khanna                                                         David O’Donnell

Dean, School of science,                                                 Director, Cell Processing

SVKM’s NMIMS University,                                        OvaScience, Inc.

Mumbai- 400 057                                                           9 Fourth Avenue, Waltham, MA 02451, USA

Email: u                              Mobile : +1-978-210-4440

Cell: +91 9324292883                             &nb

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