Details: Sperm DNA compressed into toroids are exposed by acidified Triton X-100 and DTT pre-treatment (Biomed Res Int. 2015;2015:780983). DNA damage (darker color) is revealed following simple staining (Diff-
Details: Dual use of Diff-Quik (Hema 3) for both strict morphology and chromatin integrity (Hum Reprod. 2009;24:28) can be improved (J Reprod Med. 2015;60:6) by an immediate post-stain 1-minute soak-in-water s
Details: Can you tell which image shows polymorphonuclear leukocytes(PMN) and which image shows spermatids?
Answer: The left column are PMN leukocytes or white blood cells (WBC).
Diff-Quik stain of semen. Ma
Details: Native Assessment of Sperm Ultramorphology - the microscope system developed in Altravita IVF Clinic employed both Hoffman and Nomarski contrast techniques. Resolution increase was attained with
Details: Aniline blue assay modified by adding eosin counterstain improved detection of immature sperm characterized by lysine-rich histones. Immature sperm stained dark blue while mature sperm stained red. No
Details: Sperm cells are stained using the Spermac stain and Kruger strict criteria morphology performed. The acrosome stains green while the postacrosomal nuclear region is red. Unlike PMN, spermatids are smo