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1. DNA Damage in Sperm Toroids revealed by Triton X-100 and DTT Pre-treatment before Staining.

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DNA Damage in Sperm Toroids revealed by Triton X-100 and DTT Pre-treatment before Staining. Details:
Sperm DNA compressed into toroids are exposed by acidified Triton X-100 and DTT pre-treatment (Biomed Res Int. 2015;2015:780983). DNA damage (darker color) is revealed following simple staining (Diff-

Submitted by:
Professor Philip J. Chan
Views: 2468

2. Simple Diff-Quik stain for chromatin integrity including DNA fragmentation.

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Simple Diff-Quik stain for chromatin integrity including DNA fragmentation. Details:
Dual use of Diff-Quik (Hema 3) for both strict morphology and chromatin integrity (Hum Reprod. 2009;24:28) can be improved (J Reprod Med. 2015;60:6) by an immediate post-stain 1-minute soak-in-water s

Submitted by:
Professor Philip J. Chan
Views: 2539

3. Difference between WBC and Spermatids

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Difference between WBC and Spermatids Details:
Can you tell which image shows polymorphonuclear leukocytes(PMN) and which image shows spermatids? Answer: The left column are PMN leukocytes or white blood cells (WBC). Diff-Quik stain of semen. Ma

Submitted by:
Professor Philip J. Chan
Views: 2469

4. Spermatids

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Spermatids Details:
The multinuclear cell is a result of incomplete spermatogenesis resulting in undivided spermatids as an abnormal round cell. Spermatids with cytoplasm are at the stage just before 

Submitted by:
Professor Philip J. Chan
Views: 2284

5. High Sperm DNA Damage

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High Sperm DNA Damage Details:
Sperm DNA damage in a patient exposed to environmental contaminants at work place.

Submitted by:
Kazim R. Chohan, PhD, HCLD
Views: 4104

6. Native Assessment of Sperm Ultramorphology (NASUM)

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Native Assessment of Sperm Ultramorphology (NASUM) Details:
Native Assessment of Sperm Ultramorphology - the microscope system developed in Altravita IVF Clinic employed both Hoffman and Nomarski contrast techniques. Resolution increase was attained with

Submitted by:
Alexey
Views: 5459

7. Sperm DNA Damage

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Sperm DNA Damage Details:
Sperm DNA damage evaluated with TUNEL assay. Green sperm show DNA damage.

Submitted by:
Kazim R. Chohan, PhD. HCLD
Views: 5319

8. Aniline Blue Eosin for Sperm Immaturity

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Aniline Blue Eosin for Sperm Immaturity Details:
Aniline blue assay modified by adding eosin counterstain improved detection of immature sperm characterized by lysine-rich histones. Immature sperm stained dark blue while mature sperm stained red. No

Submitted by:
Professor Philip J. Chan
Views: 13665

9. Abnormal spermatozoon

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Abnormal spermatozoon Details:
Abnormal spermatozoon Add to Favorites | Send to Friends    Description:   Spermatozoon with coiled tail and amorphous head under scanning electron microscopy, (Magnification: 1

Submitted by:
Hasan Burjaq ,MT (BSc)
Views: 6936

10. Strict Morphology Collection: Spermac Stain

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Strict Morphology Collection: Spermac Stain Details:
Sperm cells are stained using the Spermac stain and Kruger strict criteria morphology performed. The acrosome stains green while the postacrosomal nuclear region is red. Unlike PMN, spermatids are smo

Submitted by:
Professor Philip J. Chan
Views: 24400

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