Details: Sperm DNA compressed into toroids are exposed by acidified Triton X-100 and DTT pre-treatment (Biomed Res Int. 2015;2015:780983). DNA damage (darker color) is revealed following simple staining (Diff-
Details: Dual use of Diff-Quik (Hema 3) for both strict morphology and chromatin integrity (Hum Reprod. 2009;24:28) can be improved (J Reprod Med. 2015;60:6) by an immediate post-stain 1-minute soak-in-water s
Details: Can you tell which image shows polymorphonuclear leukocytes(PMN) and which image shows spermatids?
Answer: The left column are PMN leukocytes or white blood cells (WBC).
Diff-Quik stain of semen. Ma
Details: The multinuclear cell is a result of incomplete spermatogenesis resulting in undivided spermatids as an abnormal round cell. Spermatids with cytoplasm are at the stage just before
Details: Native Assessment of Sperm Ultramorphology - the microscope system developed in Altravita IVF Clinic employed both Hoffman and Nomarski contrast techniques. Resolution increase was attained with
Details: Aniline blue assay modified by adding eosin counterstain improved detection of immature sperm characterized by lysine-rich histones. Immature sperm stained dark blue while mature sperm stained red. No
Details:
Abnormal spermatozoon
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Description: Spermatozoon with coiled tail and amorphous head under scanning electron microscopy, (Magnification: 1