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Snehasish sarkar

Full Name: Snehasish sarkar
Position Sought: Embryologist and andrologist
Primary Language: Bengali, Hindi, English
Address: Kharagpur
West Bengal
Telephone: 9474183400
E-mail: Contact snehasish
 Profile and objectives
Presently, I am looking for a job in an active laboratory engaged in basic and/or applied biological sciences in the areas Biotechnology and Molecular Biology/ Cell Biology or in ART as a clinical embryologist or andrologist.
  Main resume


# Molecular Biology

DNA Extraction, Agarose Gel Electrophoresis, DNA Analysis, PCR, DNA Cloning, Restriction Digestion, Bacterial Cell Culture, Plasmid Extraction, Gene Expression, Western Blot Analysis, DNA Sequencing, Radioactive Based Hybridization Assays (Southern blot, Dot blot).

# Microscope

Stereo Microscope and Compound Microscope

# Analytical Software

Jelly-Fish, Bioinformatics tools like BLAST and Clustal Omega

# In-vitro Plant Cell and Tissue Culture

Plant Tissue Culture (Organogenesis, Somatic Embryogenesis),  Plant transformation through Agrobacterium, biolistic and in-planta

# In-vitro Insect Culture and Eggs Hatching

# Plant Breeding


# Embryology and andrology

- Oocyte collection, denuding and identification stages of oocyte maturation.

- Semen analysis and sperm preparation for IUI, IVF, ICSI.

- Surgical sperm retrieval (TESE sperm preparation), preparing retrograde ejaculation samples.

- IVF techniques (classical IVF, and ICSI) and fertilization assessment.

- Embryo culture, Embryo scoring, and Embryo transfer.

- Blastocyst culture

- Gametes and Embryo cryopreservation (vitrification, slow freezing, and thawing).  

- Determination of Sperm DNA Fragmentation (Sperm Chromatin Dispersion Test).

- Sperm HOS test.

- Quality Management.


# DNA sequencing

# Real-time polymerase chain reaction (Real-Time PCR) or qPCR

# Clinical embryology course from (IRM) Institute of Reproductive Medicine


# Participate in Author Workshop on "Scholarly Writing and Publishing" organized by the central library with the collaboration of "Springer Nature" in 2018  

Career History:

7yr. Research experience in a genetic engineering lab 


1. Clinical embryology course  from Institute of Reproductive Medicine (IRM), Kolkata, India 

2. Ph.D. (Biotechnology) 2019 from IIT-Kharagpur, India

3. M.S. (by research on biotechnology) in 2014 IIT-Kharagpur, India

4. M.Sc (Biotechnology) in 2010 from Vidyasagar  University 

5. B.Sc (Biotechnology) in 2008 from Burdwan University                                                                                                                                   

Personal details:
Interests and Hobbies:
Further Information:

1.  Snehasish Sarkar, Souri Roy, Sudip Kumar Ghosh and Asitava Basu (2018) Application of lateral branching to overcome the recalcitrance of in vitro regeneration of Agrobacterium-infected Pigeon pea (Cajanus cajan (L.) Millsp.), PCTOC (Plant Cell, Tissue and Organ Culture), Springer Nature, https://doi.org/10.1007/s11240-018-01547-6

2. Souri Roy, Snehasish Sarkar and Sudip Kumar Ghosh (2018) Somatic embryogenesis of sweet pearl millet (Pennisetum glaucum), a rich source of cellulosic biomass, Journal New Biotechnology, Volume 1113, Issue 1-3, Pages 1871-6784, Elsevier, https://doi.org/10.1016/j.nbt.2018.05.985

3. Snehasish Sarkar, Souri Roy, Sudip K.Ghosh (2018) Development of marker-free pod borer resistant Pigeon pea (Cajanus cajan). FEBS Open Bio, 8: P.07-022. https://doi:10.1002/2211-5463.12453 


1. Poster presentation on “Development of pod borer resistant marker-free transgenic plant lines of Pigeon pea" at Department of Genetics, University of Delhi, South Campus and Association of Biotechnology & Pharmacy on October 18-20, 2013, authored by Snehasish Sarkar, Souri Roy, and Sudip Kumar Ghosh.

2.  Poster presentation on “Development of marker-free pod borer resistant Pigeon pea (Cajanus cajanat 43ed Federation of European Biochemical Societies (FEBS), Prague, Czech Republic authored by Snehasish Sarkar, Souri Roy and Sudip Kumar Ghosh.

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