|To work in a progressive organization that effectively utilizes my embryology skills in advancing applications of reproductive science and to be able to work in a professionally managed environment to meet the challenges experienced by infertile couples and to deliver the result oriented performance.|
A reproduction biologist with deep understanding of oocyte/embryo, sperm genomics and assisted reproductive technologies. I am a dynamic and responsible reproduction researcher with more than six years of experience in embryology and andrology including in-vitro fertilization, embryo culture, sperm processing, cryopreservation and maintenance of various cell lines; processed almost more than 70,000 oocytes, 40,000 embryos and 4000 blastocysts. My previous work experience includes responsibility of improving the developmental competence of oocytes and embryos by synchronizing the various molecular events using the genomic tools and techniques, deciphering the male fertility markers and screening semen for optimum fertility. An animal biotechnology doctorate student with excellent academic background, international exposure and capable of performing and delivering high quality research in the field of reproduction science.
Some of the qualities that describe me:
- Dedicated, organized, proactive and responsible
- Strong work ethics and positive attitude and sincerity
- Technically sound, rich research experience
- Highly competent results oriented personality
- Effective communication and presentation skills
- National Dairy Research Institute (NDRI), Haryana: August 2015 - Present
Working as a doctorate student in the Animal Reproduction and Genomics laboratory at Animal biotechnology center (ABTC) in the research topic entitled as “Identification of the microRNAs associated with the trophectoderm cells of the blastocyst and placentome of buffaloes”
- Embryo biotechnology research and training center (EBRTC), Animal breeding Farm, Kalsi, Uttrakhand: June 2017
Worked as a mentor for practical demonstration of in-vitro maturation of OPU derived oocytes, in-vitro fertilization, in-vitro culture and embryo transfer. Set up of an IVF lab at EBRTC.
- National Dairy Research Institute (NDRI), Haryana: JULY 2013-2015
Worked as a Senior Research Fellow under NFBSAR funded project entitled “Enhancing the Developmental Competence of oocytes for better in- vitro fertilization abilities”.
- National Dairy Research Institute (NDRI), Karnal, Haryana: May 2012
Worked as an intern on the project entitled “Expression of Zygote genome activation related genes in buffalo embryos produced from different quality oocytes.
- National Bureau of Animal Genetic Resources (NBAGR), Haryana: May 2010
Worked as a trainee on project entitled ” Sequencing and phylogenetic analysis of toll like receptor 8 gene in buffaloes“
- National Dairy Research Institute (NDRI), Karnal, Haryana: June 2009
Worked as a trainee on project entitled ”Induced pluripotent stem cells”
- Bioroute Life Sciences, Karnal, Haryana: Learnt about various micro biology, molecular biology techniques.
- Media preparation, Ovum pickup (oocyte aspiration), oocyte washing and handling under stereozoom microscope.
- Oocyte, Zygote, Embryo and Blastocyst Handling and Grading.
- Assessment of oocyte maturation (CEI indexing), fertilization (PN scoring), embryo culture, blastocyst (differential staining for TE/ICM)
- Endometrial epithelial cell culture and maintenance of various cell lines
- Vitrification (Freezing & Thawing) of oocytes, embryo and blastocyst
- Trophectoderm cell culture obtained from hatched blastocysts using the matrigel/extracellular matrix system
- DNA fragmentation and Karyotyping of the cells for chromosomal analysis
- Routine semen analysis– motility, morphology, total count and sperm preparation for IUI, IVF & ICSI (Both the Swim up and density gradient method)
- Oocyte and embryo carrying from lab to another lab in portable incubator
- Microscope adjustments and experience with time-lapse technology for capturing the fertilization
- Quality control of lab equipments such as CO2 & O2 incubators.
- Genomic analysis of the various reproduction associated genes in oocytes, embryos and sperm
- CRISPR-Cas9 based genome editing and various immunohisto and immunocytochemistry staining of oocytes/embryos and sperm for analyzing their fertilizing abilities.
Doctorate of Philosophy (Animal Biotechnology) Candidate August 2015 – Present
(Thesis about to be submitted)
National Dairy Research Institute, Karnal (India)
- Grade Point Average – 8.00/10.00 CGPA
Master of Technology (Biotechnology) July 2011 – June 2013
Amity University, Noida (India)
- Grade Point Average – 8.58/10.00 CGPA
Bachelor of Technology (Biotechnology) July 2007 – June 2011
Amity University, Noida (India)
- Grade Point Average - 7.51/10.00 CGPA
Senior Secondary Examination April 2006 – March 2007
CBSE (Tagore Baal Niketan Sr. Sec. School)
- Aggregate Percentage - (75.2%)
946-H, Sector 9, U/E
Karnal (132001), Haryana, India
Cell phone: +91-8901125661, +91-9416650633
E-mail: [email protected]
|Interests and Hobbies:
- Listening to music
- Playing Badminton
- Awarded Sir William J. Fulbright-Nehru fellowship under United States India Education foundation for the year 2018-2019.
- Awarded DAAD fellowship under German academic exchange program for year 2018-2019 Leibniz Institute for Farm animal Biology, Dummerstorff, Germany.
- Awarded Rajiv Gandhi National Fellowship under University Grant Commission for the year 2014-2015
- Demonstrated IVF techniques to various researchers and doctors from SAARC countries
- Published complete IVF guidelines for livestock animals in a handsbook on livestock and Farm animals.
- Attended workshop on “CRISPR Editing in mammalian cells and embryos organized by Indo-US engineering/ editing technology initiative (GETin) program.
- Attended workshop on “Targeted Proteomics” organized by Proteomics Society, India
- Senior demonstrator of IVF technique at Animal genomics lab of NDRI, Karnal
- Editor in scientific writings
- Scientific Project Presentation designing
- Data Analysis and Interpretation
- Kumar Sandeep, Kumar Manish, Dholpuria Sunny, Sarwalia Parul*, Batra Vipul, Kumar Rakesh, De Sachinandan, Datta, Tirtha. Transient arrest of Germinal Vesicle Breakdown improved in vitro developmental potential of buffalo (Bubalus bubalis) oocytes. (2017) Journal of Cellular Biochemistry. DOI: 10.1002/jcb.26171
- Dholpuria Sunny, Kumar Manish, Kumar Sandeep, Sarwalia Parul*, Rajput Sandeep, Kumar Rakesh, De Sachinandan, Datta, Tirtha. Differential expression of newly identified long intergenic non-coding RNAs in buffalo oocytes indicating their possible role in maturation and embryonic development. (2017) Journal of Cellular Biochemistry. DOI: 10.1002/jcb.25828
- Kumar Manish, Faraji Mona, Parul*, Kumar Sandeep, Gohain Moloya, De S, Kumar Rakesh and Datta TK. (2018) Propensity in low-grade oocytes for delayed germinal vesicle breakdown compromises the developmental ability of sub-optimal grade Bubalus bubalis oocytes. Zygote. doi: 10.1017/S0967199418000321
- Chaubey GK, Kumar S, Kumar M, Sarwalia Parul*, Kumaresan, A, De S, Kumar R and Datta T K. (2018). Induced cumulus expansion of poor quality buffalo cumulus oocyte complexes by Interleukin-1beta improves their developmental ability. Journal of Cellular Biochemistry doi:10.1002/jcb.26688.
- Sunny Dholpuria, Sarwalia Parul*, Manish Kumar, Sandeep Kumar, Sandeep Rajput, Rakesh Kumar, Sachinandan De and Tirtha Kumar Datta. A novel lincRNA identified in buffalo oocytes with protein binding characteristics could holds the key for oocyte competence. (under revision) Animal Reproduction Science.