Name: Panagiota Paganopoulou
Email: [email protected]
Date of birth: 03/01/1996
2018 – 2020
University of Warwick, Doctor of Philosophy (PhD) in Life Sciences
2017 – 2018
University of Nottingham, Assisted Reproduction Technology (MMedSci) (2.1)
2014 – 2017
University of Leicester, Medical Genetics (BSc) (2.1)
2012 – 2014
Platon School, Athens, Greece, International Baccalaureate Diploma: 36 points.
LANGUAGES English: Excellent, Greek: Native
Oct 2021 - Present
Nonacus Ltd., Research and Development Technician (lead)
- Assist in the development of new products/optimisation of current products.
- NGS assay workflow (from nucleic acid to sequencer)
- Operation of sequencing technologies (Illumina).
- Nucleic acid extraction using automation/manually and quantification.
- Create SOPs, COSHH and other risk assessment documentation
- Work under ISO 13485:2016 quality management system.
Oct 2020 – Oct 2021
Nonacus Ltd., Laboratory Technician (Shift lead)
- Saliva/swab sample plating - manually and using Hamilton Microlab Star
- DNA/RNA extraction using MGI automated extractor.
- RT-qPCR Testing of SARS-CoV-2
- DNA extraction using Cell3 xtract kit – both manually and using MagCore Plus II
- DNA quantification using Qubit fluorometer, TapeStation, FLUOstar
- Adapter ligated library prep. of genomic DNA, FFPE DNA, cfDNA (using Cell3 Target kit)
- Hybridization/Capture of adapter ligated libraries and pooling for sequencing.
Jul 2018 – Oct 2020
University of Warwick, PhD candidate
Topic: Epigenetic programming of sex determination in A. freiburgensis
Techniques used: Western blot, immunohistochemistry, laser ablation, RNA interference,
confocal microscopy, RT-qPCR, RNA sequencing, Bioinformatics
Jan – March 2019
University of Leicester, 3-month miniproject
Topic: “Investigating the Role of TrpA1 in regulating the afternoon peak of locomotor behaviour under semi-natural summer conditions”.
Techniques used: Crosses, Locomotor activity assays, Imaging using DENMARK
May – July 2018
University of Nottingham, Thesis project
Topic: “The effect of paternal diet on offspring epigenetic status”.
Techniques used: RNA extraction/purification, cDNA synthesis, qPCR, ELISA
Sep – Dec 2016
University of Leicester, Dissertation project
Topic: “The effect of paracetamol on viability and antibiotic resistance of S. pneumoniae”
Techniques used: colony-forming unit (CFU) counts, preparation of chemically defined medium (CDM), gene expression analysis, growth curve generation, haemolytic assays and quantitative real- time PCR (qPCR)
University of Leicester, Internship
Topic: “The effect of different metals on resuscitation promoting factors of mycobacteria”
Techniques used: Colony forming unit (CFU) counts, colony PCR, allelic exchange mutagenesis, generating complementation vectors, gene expression analysis, SDS susceptibility testing, electrophoresis, protein purification, western blotting.
2016 – 2017
Amnesty International (University of Leicester society), Campaigner
- Participated in organisation of fundraising events & production of leaflets
- Enhanced my communication & team-working skills
2013 – 2014
Dromoi Zois Institution, Athens, Greece, Teacher
- Teaching English to immigrant children with financial needs.
- Increased my organisation and communication skills.
2012 – 2013
50+ Hellas Organisation, Athens, Greece, Teacher
- Teaching an elder student how to use the Microsoft Office software, send electronic mails and access social media.
- Found different innovative approaches to learning.
2016 – 2018
Member of the Medsin society
- Took an active role in ensuring global access to treatment in the United Kingdom
2012 – 2013
Participant of the Model United Nations (MUN) Conferences
- Chair of the Environmental Committee (Platon School, Athens)
- Delegate of the Humanitarian Committee (Costeas Geitonas, Athens)
Participant of the UNESCO student conferences
- Delegate (Jean D’ Arc School, Athens)
MS Word, Excel, PowerPoint, R studio, Command line
GraphPad Prism, Galaxy, Python, BUSCO, GAWN
Robles, P., Turner, A., Zuco, G., Adams, S., Paganopoulou, P., Winton, M., Hill, B., Kache, V., Bateson, C. and Pires-daSilva, A., 2021. Parental energy-sensing pathways control intergenerational offspring sex determination in the nematode Auanema freiburgensis. BMC Biology, 19(1).
Morgan, H., Paganopoulou, P., Akhtar, S., Urquhart, N., Philomin, R., Dickinson, Y. and Watkins, A., 2019. Paternal diet impairs F1 and F2 offspring vascular function through sperm and seminal plasma specific mechanisms in mice. The Journal of Physiology, 598(4), pp.699-715.
4-year Scholarship, MIBTP programme
3-year Chancellor’s Scholarship by University of Leicester