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Panagiota Paganopoulou

 
Full Name: Panagiota Paganopoulou
Position Sought: Embryologist/Andrologist
Primary Language:
Address: Coventry
West Midlands
United Kingdom
CV5 6HQ
Telephone: 07729902176
E-mail: Contact Panagiota
  Main resume

Name:                                      Panagiota Paganopoulou

Mobile:                                    +447729902176

Email:                                      

Nationality:                             Greek

Date of birth:                           03/01/1996

 

EDUCATION

2018 – 2020

University of Warwick, Doctor of Philosophy (PhD) in Life Sciences

2017 – 2018

University of Nottingham, Assisted Reproduction Technology (MMedSci) (2.1)

 

2014 – 2017

University of Leicester, Medical Genetics (BSc) (2.1)

 

2012 – 2014

Platon School, Athens, Greece, International Baccalaureate Diploma: 36 points.

 

 

LANGUAGES                            English: Excellent, Greek: Native

 

 

RELATED WORK

Oct 2021 - Present

Nonacus Ltd., Research and Development Technician (lead)

  • Assist in the development of new products/optimisation of current products.
  • NGS assay workflow (from nucleic acid to sequencer)
  • Operation of sequencing technologies (Illumina).
  • Nucleic acid extraction using automation/manually and quantification.
  • Create SOPs, COSHH and other risk assessment documentation
  • Work under ISO 13485:2016 quality management system.

 

Oct 2020 – Oct 2021

Nonacus Ltd., Laboratory Technician (Shift lead)

Covid workflow:

  • Saliva/swab sample plating - manually and using Hamilton Microlab Star
  • DNA/RNA extraction using MGI automated extractor.
  • RT-qPCR Testing of SARS-CoV-2

NGS workflow:

  • DNA extraction using Cell3 xtract kit – both manually and using MagCore Plus II
  • DNA quantification using Qubit fluorometer, TapeStation, FLUOstar
  • Adapter ligated library prep. of genomic DNA, FFPE DNA, cfDNA (using Cell3 Target kit)
  • Hybridization/Capture of adapter ligated libraries and pooling for sequencing.

 

Jul 2018 – Oct 2020

University of Warwick, PhD candidate

Topic: Epigenetic programming of sex determination in A. freiburgensis

Techniques used: Western blot, immunohistochemistry, laser ablation, RNA interference,

confocal microscopy, RT-qPCR, RNA sequencing, Bioinformatics

 

Jan – March 2019

University of Leicester, 3-month miniproject

Topic: “Investigating the Role of TrpA1 in regulating the afternoon peak of locomotor behaviour under semi-natural summer conditions”.

Techniques used: Crosses, Locomotor activity assays, Imaging using DENMARK

 

May – July 2018

University of Nottingham, Thesis project

Topic: “The effect of paternal diet on offspring epigenetic status”.

Techniques used: RNA extraction/purification, cDNA synthesis, qPCR, ELISA

 

Sep – Dec 2016

University of Leicester, Dissertation project

Topic: “The effect of paracetamol on viability and antibiotic resistance of S. pneumoniae

Techniques used: colony-forming unit (CFU) counts, preparation of chemically defined medium (CDM), gene expression analysis, growth curve generation, haemolytic assays and quantitative real- time PCR (qPCR)

 

August 2016              

University of Leicester, Internship

Topic: “The effect of different metals on resuscitation promoting factors of mycobacteria”

Techniques used: Colony forming unit (CFU) counts, colony PCR, allelic exchange mutagenesis, generating complementation vectors, gene expression analysis, SDS susceptibility testing, electrophoresis, protein purification, western blotting.

 

 VOLUNTARY WORK

2016 – 2017

Amnesty International (University of Leicester society), Campaigner

  • Participated in organisation of fundraising events & production of leaflets
  • Enhanced my communication & team-working skills

 

2013 – 2014

Dromoi Zois Institution, Athens, Greece, Teacher

  • Teaching English to immigrant children with financial needs.
  • Increased my organisation and communication skills.

 

2012 – 2013

50+ Hellas Organisation, Athens, Greece, Teacher

  • Teaching an elder student how to use the Microsoft Office software, send electronic mails and access social media.
  • Found different innovative approaches to learning.

 

INTERESTS

Patient Rights

2016 – 2018

Member of the Medsin society

  • Took an active role in ensuring global access to treatment in the United Kingdom   

                     

Conferences

2012 – 2013

Participant of the Model United Nations (MUN) Conferences

  • Chair of the Environmental Committee (Platon School, Athens)
  • Delegate of the Humanitarian Committee (Costeas Geitonas, Athens)

2013           

Participant of the UNESCO student conferences

  • Delegate (Jean D’ Arc School, Athens)

 

 

ADDITIONAL SKILLS

   Excellent use              

MS Word, Excel, PowerPoint, R studio, Command line

   Regular use                 

GraphPad Prism, Galaxy, Python, BUSCO, GAWN

 

 

AWARDS/PUBLICATIONS

May 2021

Robles, P., Turner, A., Zuco, G., Adams, S., Paganopoulou, P., Winton, M., Hill, B., Kache, V., Bateson, C. and Pires-daSilva, A., 2021. Parental energy-sensing pathways control intergenerational offspring sex determination in the nematode Auanema freiburgensis. BMC Biology, 19(1).

October 2019

Morgan, H., Paganopoulou, P., Akhtar, S., Urquhart, N., Philomin, R., Dickinson, Y. and Watkins, A., 2019. Paternal diet impairs F1 and F2 offspring vascular function through sperm and seminal plasma specific mechanisms in mice. The Journal of Physiology, 598(4), pp.699-715.

2018-2022                   

4-year Scholarship, MIBTP programme

2014- 2017

3-year Chancellor’s Scholarship by University of Leicester

 History
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