||Senior/Principal Embryologist, Embryology Lecturer
||Hindi, English( US), Sanskrit and Punjabi
||Dr. Vijaypat Singhania Centre of Excellence for Assisted Reproduction Technology in Livestock
|I am interested in developing new technologies in Reproduction area and work for the improvement of knowledge, skills and get a sense of satisfaction by dedicating myself completely to whatever I am assigned.|
I have extended my comprehensive academic and research experience at various International laboratories working in the field of Embryology and Reproductive techniques, with more than 5.8 years of experience in IVF, Embryonic Development, embryo freezing, sperm count and capacitation experience. I have published the International publication in the same.
1. 31st may 2016 to 25th july 2016 – worked as International "Foregin Visiting Research Officer" under the supervision of Xudong Zhao, Director and Principal Investigator of Tumor Stem - Cell laboratory at Primate Research Center, Kunming Institute of Zoology, (Chinese Academy of Sciences) East Jiaochang Road, Kunming, Yunnan 650223, China.
2. 1st March 2016 to 30th May 2016 – worked as Research Associate (RA) in Department of Science & Technology (DST) funded research project entitled “Distribution, population status and conservation genetics of cheer pheasant in himachal Pradesh” at Amity University, Noida, India.
3. 31st July 2013 to 31st Jan 2016- Research Associate (RA) in National funded research project entitled “Deciphering the mechanism of aberrant maternal recognition of pregnancy (MRP) events in Sheep and Buffalo under heat and nutritional stress”at National Dairy Research Institute, Karnal, India.
4. 05th July 2012 to 30st July 2013 - Senior Research Fellow (SRF) in National funded research project entitled “Deciphering the mechanism of aberrant maternal recognition of pregnancy (MRP) events in Sheep and Buffalo under heat and nutritional stress” at National Dairy Research Institute, Karnal, India.
5. 18th November 2011 to 31st May 2012 - Senior Research Fellow (SRF) in NAIP funded research project entitled “Elucidating the physiological and genomic regulation process of oocytes maturation, follicular development and embryogenesis in buffalo”at National Dairy Research Institute, Karnal, India.
6. Jan 2010 to July 2010 – Master Research Project “Estimation of Genetic Diversity in Indigenous Cattle Breeds revealed by Mitochondrial DNA D-loop sequence variation” from National Bureau of Animal Genetic Resourses (NBAGR), Karnal, Haryana, India.
7. July 2009 to August 2009 – Industrial Trainee at IND-Swift Laboratory Limited, Mohali –Chandigarh, India.
Embryology & Reproductive Techniques:
Female egg’s cell Aspiration, Collection of Oocytes,Denudation of oocytes with machnically and enzymmatically, oocytes grading, Morphology detection,IVM (in vitro maturation), IVF (in vitro fertilization) and IVC (in vitro culture) of Buffalo,Sheep, Mouse, etc.Sperm morphology evalution, sperm count and capacitation, Immune- Histochemical Processing of oocytes as BCB (Brilliantcresyl blue) & Hoechest staining of buffalo oocyte, Virus Culture and Embryo documentating at different cell stages till blastocyst production.
Managing IVF – Culture lab
Responsible for quality control of lab equipments.
Routine semen analysis/oocytes morphology analysis, oocytes denudation.
Perform IVM,IVF, IVC
Sperm preparation for IVF,
Cryopreservation of Embryos/oocytes, Embryo freezing, Embryos thawing and loading,
Maintain the stock of all the materials and media requirements of the lab.
Maintain the Co2 Incubator, Micromanipulator and zoom sterio Microscope
Maintain the daily logs of equipments and instruments used in the lab.
Embryology, Animal Cell Biology and Developmental Rate Studies:
Female egg’s cell aspiration, oocytes morphology/grading detection, collection of oocytes from buffalo, mouse, sheep etc, oocytes searching from follicular fluid under zoom stereo microscope, oocytes/ embryos in-vitro development media preparation, IVM (in- vitro maturation), IVF (in -vitro fertilization), IVC (in- vitro culture) of Buffalo, oocytes/sperm RNA extraction, oocytes/sperm cDNA synthesis, mRNA abundance study in oocytes, ovary’s, sperm, COC’s, Immune- Histochemical staining processing of oocytes, different kind of staining and hormonal/ protein treatment, BCB staining (Brilliantcresyl blue), Hoechest staining of buffalo oocyte, sperm quality evaluation, sperm count and capacitation, Intracytoplasmic Sperm Injection (ICSI), Stem – cell line culture, Embryokines studies, embryo developmental rate studies, virus Culture and Embryo harvesting/documentating at different cell stages till blastocyst production, Heat –stress treatment for oocytes/embryos Methods for studies of cell metabolism, to quick design different types of experiment and statistical analysis of results, etc
Molecular Biology & Genomics:
DNA & RNA isolation from various animal samples, Agarose gel electrophoresis, End point - PCR, Reverse Transcriptase-PCR, Nested PCR and Real-Time PCR, analysis of gene expression, accurate normalization of quantitative Real Time Data by geometric mean methods, cDNA synthesis, BrUTP incorporation, in – situ hybridization, gene transfer techniques viz. electoporation/Micromanipulator, ATPase assay, Glutathione assay, JC-1 Staining for mitochondrial gene stress status studies, SNPs detection, Multiple sequence result analysis, Phylogenetic Analysis,etc
Isolation of microorganisms from various sample types like air, soil and water, gram staining, Negative staining, Acid fast staining, enumeration of microorganism, measurement of fungal and bacterial growth, MBRT test, etc.
ELISA, Radial and double immune-diffusion, other serodiagnostic techniques as Blood testing, etc.
Estimation of Protein and Carbohydrate, W.B.Cs, R.B.Cs and TL(total leukocyte) counting,Quantitative estimation of DNA &RNA, etc.
Research Training Supervision: Keen mentoring, advising, constant supervision for the young Researcher denotes teaching, supervising and persistent encouragement of several M.Sc./MV.Sc./ M.Tech/ B.Tech students thesis and Research Project on embryology.
1. Master of Science in Biotechnology (Honors.) - (2008 - 2010) from Lovely Professional University (Punjab) http://www.lpu.in/
2. Bachelor of Science in Biotechnology (Honors.)–(2005 - 2008) From Bundelkhand University (Jhansi) https://www.bujhansi.org/index.aspx
M.Sc. Biotechnology (Honors) Project:
Jan 2010 to July 2010 - “Estimation of Genetic Diversity in Indigenous Cattle Breeds revealed by Mitochondrial DNA D-loop sequence variation” from National Bureau of Animal Genetic Resourses (NBAGR), Karnal, Haryana, India.
1. Standardized DNA extraction protocols and PCR cycling conditions for Mitochondrial DNA D- loop region in indigenous cattle breeds.
2. Established phylogenetic relationship among different cattle breeds using D-loop sequencing data.
Mr. Manish Kumar, (Assistant Lab. Manager- Embryology)
BovaGenix, Pune (Maharashtra)
Contact: +91-8950171930, +91-8318059319
Skype ID: GeniousGeneM
E-mail: [email protected] Alt.E-mail: [email protected]
Web link: http://www.ivf.net/ivf/manish-kumar-o9082.html
|Interests and Hobbies:
Academic Awards / Research Scholarships:
1. Chinese Government Scholarship - International Foreign Visiting Researcher to work with Primate Research Centre, Kunming Institute of Zoology, (Chinese Academy of Sciences) Kunming, Yunnan 650223, China. - 31st may 2016 to 25th July 2016.
2. Amity University, Noida, India - Department of Science & Technology (DST) Research Associate ship (RA) - March 2016 to May 2016.
3. NDRI-National Fund, India Indian Council of Agricultural Research (ICAR) Research Associate ship (RA) - July 2013 to Dec 2015.
4. NDRI-National Fund, India Indian Council of Agricultural Research (ICAR) Senior Research Fellow (SRF) - July 2012 to July 2013.
5. NDRI- National Agriculture Innovative Programme (NAIP) India Indian Council of Agricultural Research (ICAR) Senior Research Fellow (SRF) -Nov 2011 to May 2012.
6. 2005- Secured All India Rank 20th in Graduate Entrance Test conducted for Bachelor of Science in Biotechnology with Honours Conducted by Bundelkhand University, Jhansi (Premium University in INDIA).
Professional Credentials: Member – Ship of Professional Societies:
Active member of International Society of Zoological Sciences, Institute of Zoology Beijing, China (Membership ID 941-I)
Microsoft office- MS excel, PowerPoint MS Word and expertise in web browsing.
Strengths: Self-Dependable, very good mentor, reliable, hard-working, conscientious, honest and a good team member.
(1) Sandeep Kumar, Manish Kumar*, Sunny Dholpuria, Parul Sarwalia, Vipul Batra,Sachinandan De, Rakesh Kumar, and Tirtha Kumar Datta (2017) Transient Arrest of Germinal Vesicle Breakdown Improved In Vitro Development Potential of Buffalo (Bubalus Bubalis) Oocytes. Journal of Cellular Biochemistry. DOI: 10.1002/jcb.26171. (Impact factor: 3.446)
(2) Suman Choudhary, A. Kumaresan, Manish Kumar*, Hrudananda Malik, Sudarshan Kumar, Jai K. Kaushik, Tirtha K. Datta, and Ashok K. Mohanty (2017) Effect of recombinant and native buffalo oviductin on sperm functions and in vitro embryo development: A comparative study. Journal of Animal Science and Biotechnology. DOI: 10.1186/s40104-017-0201-5. (Impact factor: 2.052)
(3) Dholpuria Sunny, Kumar Manish*, Kumar Sandeep, Sarwalia Parul, Rajput Sandeep, Kumar Rakesh, De Sachinandan, Datta, Tirtha. (2016) Differential expression of newly identified long intergenic non-coding RNAs in buffalo oocytes indicating their possible role in maturation and embryonic development. Journal of Cellular Biochemistry. DOI: 10.1002/jcb.25828 (Impact factor: 3.446)
(4) A Jain, T Jain, P Kumar, M Kumar*, S De, M Gohain, R Kumar and T K Datta. (2015) FSH-induced rescue of cumulus cell apoptosis and enhanced development ability of buffalo oocytes. Domest Anim Endocrinol. DOI: 10.1016/j.domaniend.2015.10.007. (Impact factor: 1.613)
(5) Parveen Kumar, Arpana Verma, Manish Kumar*,Sachinandan De, Rakesh Kumar & Tirtha Kumar Datta. (2015) Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition. Journal of Assisted Reproduction and Genetics. DOI 10.1007/s10815-014-0418-6 (Impact factor: 1.585)
(6) Devender Singh, Manish Kumar*, Arpana Verma, rakesh kumar, Sachinandan De and Tirtha Datta (2017) Buffalo oocytes exposed to heat stress did cleave but failed to crawl through morula to blastocyst transition. (under revision) Journal of Thermal Biology.(Impact factor: 1.621)
(7) Sunny Dholpuria, Manish Kumar*, Sandeep Kumar, Sandeep Rajput, Rakesh Kumar, Sachinandan De and Tirtha Kumar Datta (2017) A novel lincRNA identified in buffalo oocytes with protein binding characteristics could holds the key for oocyte competence. (under revision) Animal Reproduction Science. (Impact factor: 1.377)
(8) Manish Kumar*, Mona Faraji, Parul, Sandeep Kumar, Moloya Gohain, Sachinandan De, Rakesh Kumar and T K Datta. (2017) Delayed propensity of Germinal vesicle breakdown (GVBD) in BCB negative buffalo oocytes and their compromised development ability. (under revision) Theriogenology. (Impact factor: 1.838)
(9) Chaubey Gaurav, Kumar Sandeep, Manish Kumar*, Rakesh Kumar & Tirtha Kumar Datta. (2017) Induced cumulus expansion of poor quality buffalo cumulus oocyte complexes by Interleukin1beta improves their developmental ability. Status- paper (submitted) Molecular Reproduction & Development.(Impact factor: 2.141)
Scientific Poster/Oralpresentation/Abstract Publication:
- XII Agricultural science congress (India) topic on “unstainable Livelihood Security of Smallholder Farmers” National Dairy Research Institute, Karnal, India (Poster presentation, abstract submission and oral Presentation on following topics which are given below), 5-6 Feb, 2015 (web ref. - http://agricongress2015.in/)
- Manish Kumar*, Mona Faraji, Parul, Sandeep Kumar, Devender Singh, Rakesh Kumar and T.K.Datta (2015) Delayed germinal vesicle breakdown propensity of inferior quality buffalo oocytes.(Ref. ASC2015ABS1418298096)
- Devender Singh, Manish Kumar*, Megha Saini, S.De and T.K.Datta (2015) Development of an in – Vitro heat stress model for buffalo oocytes stuck to crawl through Morula to Blastocyst transition. (Ref. ASC2015ABS1418640239)
- Devender Singh, Manish Kumar*, Parul, Moloya Gohain, S.De and T.K.Datta (2015) Buffalo oocytes exposed to heat stress did cleave but failed to crawl through Morula to blastocyst transition. (Ref. ASC2015ABS1419920241)
- Sunny Dholpuria, Sandeep Rajput, Manish Kumar*, Parul, Sachinandan De, Rakesh Kumar and T.K.Datta (2015) Unique non –coading RNAs identified in buffalo oocytes which could be crucial for their developmental competence.(Ref. ASC2015ABS1418387766)
- Sandeep Kumar, Moloya Gohain, Sunny Dholpuria, Manish Kumar*, Parul, Asmita, Sachinandan De, Rakesh Kumar and T.K.Datta (2015) Synchronizing the buffalo oocytes for germinal vesicle break down (GVBD) can enhance their developmental competence. (Ref. ASC2015ABS1418387800)
- Manish Kumar*, Mona Faraji, Parul, Sandeep Kumar, Devender Singh and T.K.Datta (2014) “National Conference on Recent Developments in Science and Technology” at Biotech Department HPU Shimla, India (web ref. - http://www.isrsindia.com/)
Conference/Workshop/Summer School Attended:
- “Update on Screening of Breast & Cervical Cancer” at Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow - India, 4 Feb, 2013.
- International Symposium Indo - UK Genetic Education Forum – “ Current Trends in Genetic & Genomic Medicine” at Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, India, 31 Jan 2013
- International Symposium on “Recent Advances in Biochemistry” at Ers’s Lucknow Medical College & Hospital, Lucknow-India, 30 Jan,2013.
- “International Symposium on Animal Models of Human Diseases” – Second Annual Conference of the Indian Academy of Biomedical Sciences (IABS) at Department of Biochemistry, King George’s Medical University, Lucknow-India 29 Jan, 2013.
- International Symposium on “Reproductive Biotechnology” held at National Dairy Research Institute, Karnal –Haryana-India during 30-31 Jan, 2012.
- National Science Day Workshop on “Awareness about Biodiversity and its conservation using Biotechnological Tools” held at National Bureau of Animal Genetic Recourses (NBAGR), Karnal-India, 28 Feb, 2009.
- International Knowledge Exchange programme in life sciences education and research participated in the seminar KEPLER – 2007 India, held at J. C. Bose Institute of Life sciences during 16 -17 Jan, 2007.