Principles of vitrification workshop
Dr. Yona Barak, 11 October 2011 You are invited to join the hands-on workshop organized by
The workshop will introduce the participants to vitrification of oocytes and embryos in open and closed system in slush of LN2. During the theoretical session will include: The physical principles of Vitrification, introduction to minimum volume methods, cooling rates and different vitrification devices *
09:00- 12:00
Theoretical Session:
Principles on Vitrification of Oocytes and Embryos
Practical Aspects of Human Oocytes Vitrification
12:00-13:00 Lunch 13:00-17:00 Hands-on Vitrification of Oocytes/Embryos
During the session the participants will be introduced to the following:
* The usage of slush (VitMaster) and irradiation of LN
* Cryo-microscope as a tool for the evaluation of vitrification outcome
* Trouble shooting during cooling and warming: Solution effect ; How to produce a small drop containing oocytes; Open vs Closed system; LN vs. Slush; vitrification; crystallization, de-vitrification, fracturing and recrystallization phenomenons
Each participant will try to vitrify oocytes using Sealed SOPS. Else, each participant will have silica beads for practicing in their own facilities. 17:30 COGI Opening Keynote lecture * The existing of the workshop is dependent upon the number of participants. |
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