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calcium ionophore powder shelf life

By: SAI, MALPANI INFERTILITY CLINIC PVT. LTC. 18 October 2014

HI GUYS,

HAS ANYBODY USED 4-BROMO CALCIUM IONOPHORE (A 23187) (SIGMA B-7272) FOR ASSISTED OOCYTE ACTIVATION (AOA)?

WE DID ASSISTED OOCYTE ACTIVATION WITH CALCIUM INOPHORE FOR ONE OF OUR PATIENT WHO WAS GLOBOZOOSPERMIC (100% ROUND HEAD SPERMS, NO ACROSOME).

WE GOT 50 % FERTILISATION WITH TOP QUALITY EMBRYOS ON DAY 2 (4-CELL GRADE A). WHICH WERE TRANSFERRED AND THE PATIENT CONCEIVED.

MY QUESTION IS ABOUT THE SHELF LIFE OF CALCIUM IONOPHORE.

AS THERE IS NO EXPIRY DATE ON THE CALCIUM IONOPHORE POWDER VIAL, HOW LONG CAN ONE USE IT?

NOTE : THE POWDER IS DILUTED WITH CULTURE MEDIUM TO ACHIVED A CONCENTRATION OF 5UM, AS THE PROTOCOL SAYS THAT WE SHOULD KEEP THE INJECTED EGGS IN 5uM 4-BROMO CALCIUM IONOPHORE FOR ASSISTED OOCYTE ACTIVATION.

 

kINDLY PROVIDE SOME INFORMATION REGARDING THIS, IF ANYONE HAS USED IT.

THANK YOU IN ADVANCE

 

 

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Responses (3)
Response from Dr. M. D. Kini. MB.,FRCOG (UK) 12 September 2016
Please contact Dr. Murthy at Miracle whop can answer your question.
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Response from Khaled Ali 11 September 2016
I had used calcium ionophor powder as following 1- dissolved Pawder in 2ml 100%ethanol (stock solution) preserved in refrigerator 2-for oocyte activation we add 10 microliter from stock solution to 1ml from culture media incubated with CO2 6% and used as activation media 3- add the oocytes immediately after injection to activation media for 10minutes in incubator CO2 6% 4-rense the oocyrtes from activation media and transfer it to culture media 5- I have no expiry date for stock media I had got agreat results. Now I used gynemed cult active media for activation of oocytes
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Response from Dayanidhi Kumar 20 October 2014
The Shelf life of 4-BROMO CALCIUM IONOPHORE (A 23187) (SIGMA B-7272)is 5 years in powder form at 2-8 °C. Once It is diluted with culture media, must be used freshly to get better result. The powder can be dissolved in DMSO (stock solution)and stored at -20°C for 3 months. At the time of ASSISTED OOCYTE ACTIVATION, it should be added to culture medium to achieve a concentration of 5UM. Please refer the below link for more information. https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/3/c7522dat.pdf
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